Methane- Producing Anaerobic Sewer Biofilms
نویسندگان
چکیده
Stratified Microbial Structure and Activity in Sulfideand 1 MethaneProducing Anaerobic Sewer Biofilms 2 3 Jing Sun, Shihu Hu, Keshab Raj Sharma, Bing-Jie Ni, Zhiguo Yuan* 4 5 1 Advanced Water Management Center, The University of Queensland, St. Lucia, 4072, 6 Queensland, Australia; 7 2 CRC for Water Sensitive Cities, PO Box 8000, Clayton, Victoria, 3800, Australia 8 9 *Corresponding author 10 Email: [email protected] 11 Phone: +61 (0)7 3365 4374 12 Fax: +61 (0)7 3365 4726 13 14 15 ABSTRACT 16 Simultaneous production of sulfide and methane by anaerobic sewer biofilms has 17 recently been observed, suggesting that sulfate-reducing bacteria (SRB) and 18 methanogenic archaea (MA), microorganisms known to compete for the same substrates, 19 can coexist in this environment. This study investigated the community structures and 20 activities of SRB and MA in anaerobic sewer biofilms (average thickness of 800 μm) 21 using a combination of microelectrode measurements, molecular techniques and 22 mathematical modelling. It was seen that sulfide was mainly produced in the outer layer 23 of the biofilm, between 0 300 μm, which is in good agreement with the distribution of 24 SRB population as revealed by cryosection fluorescence in situ hybridization (FISH). 25 AEM Accepts, published online ahead of print on 5 September 2014 Appl. Environ. Microbiol. doi:10.1128/AEM.02146-14 Copyright © 2014, American Society for Microbiology. All Rights Reserved.
منابع مشابه
Changes in Microbial Biofilm Communities during Colonization of Sewer Systems.
The coexistence of sulfate-reducing bacteria (SRB) and methanogenic archaea (MA) in anaerobic biofilms developed in sewer inner pipe surfaces favors the accumulation of sulfide (H2S) and methane (CH4) as metabolic end products, causing severe impacts on sewerage systems. In this study, we investigated the time course of H2S and CH4 production and emission rates during different stages of biofil...
متن کاملOptimization of intermittent, simultaneous dosage of nitrite and hydrochloric acid to control sulfide and methane productions in sewers.
Free nitrous acid (FNA) was previously demonstrated to be biocidal to anaerobic sewer biofilms. The intermittent dosing of FNA as a measure for controlling sulfide and methane productions in sewers is investigated. The impact of three key operational parameters namely the dosing concentration, dosing duration and dosing interval on the suppression and subsequent recovery of sulfide and methane ...
متن کاملAcido solfidrico nella letteratura internazionale
Sulfate-reducing bacteria (SRB) are widely existed in oil production system, and its H2S product inhibits rhamnolipid producing bacteria. In-situ production of rhamnolipid is promising for microbial enhanced oil recovery. Inhibition of SRB, removal of H2S and production of rhamnolipid by recombinant Pseudomonas stutzeri Rhl were investigated. Strain Rhl can simultaneously remove S(2-) (>92%) an...
متن کاملModeling the pH effect on sulfidogenesis in anaerobic sewer biofilm.
Wastewater pH affects the transfer of H2S from liquid to gas and hence impacts on the extent of odour and corrosion. Sewage pH may change significantly in sewer networks due to, for example, the fermentation of organic compounds, particularly when the hydraulic retention time is long, the discharge of trade wastes that are organic-rich or with extreme pH, and the intentional addition of alkali ...
متن کاملReversing methanogenesis to capture methane for liquid biofuel precursors
BACKGROUND Energy from remote methane reserves is transformative; however, unintended release of this potent greenhouse gas makes it imperative to convert methane efficiently into more readily transported biofuels. No pure microbial culture that grows on methane anaerobically has been isolated, despite that methane capture through anaerobic processes is more efficient than aerobic ones. RESUL...
متن کامل